Ultra-LEAF™ Purified anti-mouse CD3 100254 from BioLegend

Product Specs
ItemUltra-LEAF™ Purified anti-mouse CD3
CompanyBioLegend
Price $1,408.00Supplier Page View Company Product Page
Catalog Number100254
Quantity25 mg
ApplicationsFC - Quality tested, IHC-F, IP, ICC - Reported in the literature, not verified in house
Conjugate/TagUnconjugated, Ultra Low Endotoxin Azide Free
Format0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative
ReactivityMouse
TargetCD3
IsotypeRat IgG2b, kappa
Clone17A2
HostRat
Antibody TypePrimary
ImmunogenγδTCR-positive T-T hybridoma D1
NCBI Full Gene NameCD3 antigen, gamma polypeptide
NCBI Gene AliasesCtg-3, Ctg3, T3g
ClonalityMonoclonal
ConcentrationThe antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool

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BioLegend

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San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Inhibition of bruton’s tyrosine kinase directly alleviates pathogenic CD4+ T cell response via PPAR-γ signaling in autoimmune uveitisJournal of NeuroinflammationNovember 27, 2025Zhiqiang Xiao, Zhuang Li, Wenjie Zhu, Guanyu Chen, Xiaoshuang Luo, Zhiqing Mai, Minzhen Wang, Haixiang Huang, Zuoyi Li, Yuxun Shi, et al.purity confirmed by flow cytometry (> 95%). Cells were seeded at 2 × 10^5 cells/well in anti-CD3 (CAT#100239, BioLegend, USA) pre-coated 96-well plates and cultured under Th17, Th1, or Treg polarizing conditions

(2) Neutrophil extracellular traps exacerbate inflammatory arthritis by inhibiting γδ Treg cell differentiation via the AIM2 inflammasomeRedox BiolSeptember 30, 2025Yipeng Zeng, Jiajie Lin, Zipeng Xiao, Zhikun Li, Guan Zheng, Yi Zhou, Qibo Li, Chenying Zeng, Zepeng Su, Wenhui Yu, et al.vein injection. Additionally, the mice were injected intravenously with 0.1 μg/g anti-CD3 (BioLegend, #100239) 24 h before NET administration. Forty-eight hours later, blood and spleens were collected for analysis. 2.4

(3) AR+TREM2+ macrophage induced pathogenic immunosuppression promotes prostate cancer progressionNat CommunJuly 29, 2025Qiaohua Wang, Yongjian Wu, Yili Long, Rongna Li, Yu Shi, Yanfen Zheng, Xiaohui Chen, Xiang Li, Yihong Zhou, Xi Huang, et al.(Corning, 7007), followed by in vitro activation using anti-CD3 and anti-CD28 antibodies (BioLegend, 100253/102132). In vitro-induced WT or TREM2 KO BMDMs and RM1 cells were co-cultured with the splenocytes.

(4) Double-negative T cells in combination with ursodeoxycholic acid ameliorates immune-mediated cholangitis in miceBMC MedApril 7, 2025Chunpan Zhang, Guangyong Sun, Hua Jin, Yunxiong Wei, Shimeng Zheng, Xiyu Wang, Xinyan Zhao, Dong Zhang, Jidong Jiasequencing and biological function analysis WT and Fxr−/− DNT were stimulated with anti-mouse CD3 (Cat#: 100256, Biolegend, USA) and anti-mouse CD28 (Cat#: 117003, Biolegend, USA) antibodies, with or without UDCA

(5) PKN2 enhances the immunosuppressive activity of polymorphonuclear myeloid-derived suppressor cells in esophageal carcinoma by mediating fatty acid oxidationMolecular medicine (Cambridge, Mass.)March 11, 2025Fu X, Zhang G, Hou Z, Fu T, Cui G.former were transfected with lentivirus. The co-culture system was supplemented with anti-CD3 (10 µg/mL, 100253, BioLegend, San Diego, CA, USA) and anti-CD28 (5 µg/mL, 102121, BioLegend) to activate T cells, whereas

(6) STING Deficiency Promotes Th17-Like Tfh to Aggravate the Experimental Autoimmune UveitisInvest Ophthalmol Vis SciMarch 5, 2025Zhuang Li, Xiuxing Liu, Zuoyi Li, Zhiqiang Xiao, Guanyu Chen, Yangyang Li, Jun Huang, Yunwei Hu, Haixiang Huang, Wenjie Zhu, et al.0.2 million/well) were seeded into 96-well-plate which had been coated by 10 µg/mL anti-CD3 (catalog #100239; Biolegend, San Diego, CA, USA). Then, naïve CD4+ T cells were cultured with 10% fetal bovine serum

(7) MyD88 determines T cell fate through BCAP-PI3K signalingThe Journal of ImmunologyMarch 1, 2025Abraham L. Bayer, Zoie Magri, Hayley Muendlein, Alexander Poltorak, Pilar Alcaidemillion cells / mL and differentiated to Th1 cells in the presence of anti-CD3 (2.5 μg/mL, Biolegend 100253), anti-CD28 (1 μg/mL, Biolegend 102102), IL-12 (10 ng/mL, Peprotech 210-12), IL-2 (25U/mL, Miltenyi,

(8) Purinergic signaling by TCRαβ+ double-negative T regulatory cells ameliorates liver ischemia–reperfusion injurySci Bull (Beijing)January 30, 2025Hua Jin, Mingyang Li, Xiyu Wang, Lu Yang, Xinjie Zhong, Zihan Zhang, Xiaotong Han, Jingjing Zhu, Mengyi Li, Songlin Wang, et al.splenocytes by FACS. Activated murine natural DNT (aDNT) were stimulated with 5 μg/ml of anti-CD3 (100253, Biolegend, San Deigo, USA) and 2 μg/ml of anti-CD28 antibodies (102121, Biolegend) for 2–4 d. Human

(9) Flotillin-2 dampens T cell antigen sensitivity and functionalityJCI insightDecember 20, 2024Moon S, Zhao F, Uddin MN, Tucker CJ, Karmaus PW, Fessler MB.Naive CD4+ or CD8+ T cells were stimulated with various concentrations of plate-bound αCD3 (BioLegend, 100253) and a fixed concentration of soluble αCD28 (BioLegend, 102121; 1 μg/mL) antibodies for different time

(10) Cytotoxic T cells drive doxorubicin-induced cardiac fibrosis and systolic dysfunctionNature Cardiovascular ResearchJuly 17, 2024Abraham L. Bayer, Maria A. Zambrano, Sasha Smolgovsky, Zachary L. Robbe, Abul Ariza, Kuljeet Kaur, Machlan Sawden, Anne Avery, Cheryl London, Aarti Asnani, et al.2 million cells per ml and activated in the presence of anti-CD3 antibody (2.5 μg ml−1; Biolegend, 100253) and anti-CD28 antibody (1 μg ml−1; Biolegend, 102102), for 3 days then collected for experiments. For

(11) Heat shock protein 90α reduces CD8+ T cell exhaustion in acute lung injury induced by lipopolysaccharideCell Death DiscoveryJune 13, 2024Lei Yan, Yumei Chen, Yilin Yang, Yi Han, Chaoyang Tong(BioLegend, 575402) in 12-well plates, which were precoated with 5 μg/mL anti-mouse CD3 (BioLegend, 100239) and 0.5 μg/mL anti-mouse CD28 Abs (BioLegend, 302941). Then CD8+ T cells were incubated with 5 μM DMSO

(12) Association of TRAIL receptor with phosphatase SHP-1 enables repressing T cell receptor signaling and T cell activation through inactivating LckJournal of biomedical scienceMarch 27, 2024Chyuan IT, Liao HJ, Tan TH, Chuang HC, Chu YC, Pan MH, Wu CS, Chu CL, Sheu BC, Hsu PN.(catalog no. Z03367) was purchased from GenScript (Piscataway, NJ). Stimulatory anti-CD3 (catalog no. 100253) and anti-CD28 (catalog no. 102121) antibodies were purchased from BioLegend (San Diego, CA). The antibodies

(13) Impaired T cell IRE1α-XBP1 signaling directs inflammation in experimental Heart Failure with Preserved Ejection FractionJournal of Clinical InvestigationDecember 15, 2023Sasha Smolgovsky, Abraham L. Bayer, Kuljeet Kaur, Erin Sanders, Mark Aronovitz, Mallory E. Filipp, Edward B. Thorp, Gabriele G. Schiattarella, Joseph A. Hill, Robert M. Blanton, et al.million cells/mL in complete media and differentiated in the presence of αCD3 (2.5 μg/mL; BioLegend, 100253) and αCD28 (1 μg/mL; BioLegend, 102102) for 3 days at 37°C, then harvested for adoptive transfer experiments.

(14) Systematic investigation of mitochondrial transfer between cancer cells and T cells at single-cell resolutionCancer CellOctober 2, 2023Hongyi Zhang, Xuexin Yu, Jianfeng Ye, Huiyu Li, Jing Hu, Yuhao Tan, Yan Fang, Esra Akbay, Fulong Yu, Chen Weng, et al.(Stem Cell Technologies, Cat# 19853). Isolated T cells were activated with 1ug/mL anti-CD3/CD28 (Biolegend, Cat#100239 and Cat#102115) antibodies for 24h. Activated CD8+ T cells were then stained with 100 nM MitoTracker™

(15) The Flavonoid Naringenin Alleviates Collagen-Induced Arthritis through Curbing the Migration and Polarization of CD4(+) T Lymphocyte Driven by Regulating Mitochondrial FissionInternational journal of molecular sciencesDecember 23, 2022Jiang YP, Wen JJ, Zhao XX, Gao YC, Ma X, Song SY, Jin Y, Shao TJ, Yu J, Wen CP.

(16) Impaired Treg-DC interactions contribute to autoimmunity in leukocyte adhesion deficiency type 1JCI insightDecember 22, 2022Klaus T, Wilson AS, Vicari E, Hadaschik E, Klein M, Helbich SSC, Kamenjarin N, Hodapp K, Schunke J, Haist M, et al.

(17) IL-33 induces thymic involution-associated naive T cell aging and impairs host control of severe infectionNature communicationsNovember 12, 2022Xu L, Wei C, Chen Y, Wu Y, Shou X, Chen W, Lu D, Sun H, Li W, Yu B, et al.

(18) Systemic Delivery of mPEG-Masked Trispecific T-Cell Nanoengagers in Synergy with STING Agonists Overcomes Immunotherapy Resistance in TNBC and Generates a Vaccination EffectAdvanced science (Weinheim, Baden-Wurttemberg, Germany)November 1, 2022Shen M, Chen C, Guo Q, Wang Q, Liao J, Wang L, Yu J, Xue M, Duan Y, Zhang J.antibodies, anti‐human CD3 antibodies, and anti‐human PD‐L1 antibodies were purchased from Biolegend (100254, 124339, 317348, 329746). CDA (2′3′‐c‐di‐AM (PS)2 (Rp, Rp)) was purchased from InvivoGen (tlrl‐nacda2r‐01).

(19) Murine RAW Macrophages Are a Suitable Model to Study the CD3 Signaling in Myeloid CellsCellsMay 13, 2022Ocaña-Guzmán R, Ramón-Luing LA, Rodríguez-Alvarado M, Voss TD, Fuchs T, Chavez-Galan L.TruStain FcX™ (anti-mouse CD16/32) Antibody,BioLegend,93,101320 Ultra-LEAF(TM) Purified anti-mouse CD3 antibody,BioLegend,17A2,100239 Ultra-LEAF Purified Rat IgG2b kappa Isotype Ctrl rat,BioLegend,RTK4530,400643 anti NF kappa B p65,Santa

(20) CD137 (4-1BB) costimulation of CD8⁺ T cells is more potent when provided in cis than in trans with respect to CD3-TCR stimulationNature communicationsDecember 15, 2021Otano I, Azpilikueta A, Glez-Vaz J, Alvarez M, Medina-Echeverz J, Cortés-Domínguez I, Ortiz-de-Solorzano C, Ellmark P, Fritzell S, Hernandez-Hoyos G, et al.

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Ultra-LEAF™ Purified anti-mouse CD3 from BioLegend

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Ultra-LEAF™ Purified anti-mouse CD3 from BioLegend

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