Immunofluorescence staining of Syndecan 4 in Hela cells or mouse splenocytes. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Syndecan 4 Recombinant Rabbit Monoclonal Antibody (47) (Product # MA5-29783, 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

Immunohistochemical staining of mouse Syndecan 4 in mouse stomach with Syndecan 4 Recombinant Rabbit Monoclonal Antibody (47) (Product # MA5-29783, 1:200, formalin-fixed paraffin embedded sections).

Immunocytochemistry analysis of Syndecan 4 in mouse splenocytes using Syndecan 4 Recombinant Monoclonal (Product # MA5-29783). Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rSyndecan 4 antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.