Immunohistochemistry-Immunofluorescence analysis of ZP2 was performed in mouse oophoroma tissue using ZP2 Polyclonal Antibody (Product # PA5-87653) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Immunohistochemistry-Immunofluorescence analysis of ZP2 was performed in mouse oophoroma tissue using ZP2 Polyclonal Antibody (Product # PA5-87653) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of ZP2 in rat ovary cells. Samples were incubated with ZP2 Polyclonal antibody (Product # PA5-87653) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of ZP2 in extracts of various cell lines. Samples were incubated with ZP2 Polyclonal antibody (Product # PA5-87653) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.