Immunocytochemistry-Immunofluorescence analysis of FSTL1 was performed in NIH/3T3 cells using FSTL1 Polyclonal Antibody (Product # PA5-90705) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of FSTL1 in extracts of various cell lines. Samples were incubated with FSTL1 Polyclonal antibody (Product # PA5-90705) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.