Immunocytochemistry-Immunofluorescence analysis of TSLP was performed in U-2 OS cells using TSLP Polyclonal Antibody (Product # PA5-89013) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of TSLP in extracts of various cell lines. Samples were incubated with TSLP Polyclonal antibody (Product # PA5-89013) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.