For immunofluorescence analysis, MCF7 cells were fixed and permeabilized for detection of endogenous SLIRP using Anti-Citrate synthase Recombinant Rabbit Monoclonal Antibody (Product # 703632, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of SLIRP protein (green), Panel b) is stained for nuclei (blue) using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). Panel c) represents mitochondrial staining using MitoTracker® Red CMXRos (Product # M7512). Panel d) is a composite image of Panels a, b and c clearly demonstrating co-localization of SLIRP with mitotracker which specifically binds to the mitochondria. Panel e) represents control cells without primary antibody to assess background. The images were captured at 60X magnification.