Immunofluorescence staining of ORP150 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with HYOU1 Recombinant Rabbit Monoclonal Antibody (201) (Product # MA5-29334, 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.

HYOU1 Immunoprecipitation using: Lane A: 0.5 mg MCF-7 Whole Cell Lysate, Lane B: 0.5 mg 293T Whole Cell Lysate, Lane C: 0.5 mg Hela Whole Cell Lysate 2 µL with HYOU1 Recombinant Rabbit Monoclonal Antibody (201) (Product # MA5-29334) and 15 µL of 50 % Protein G agarose. Primary antibody: HYOU1 Recombinant Rabbit Monoclonal Antibody (201), at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 111 kDa. Observed band size: 111 kDa.