Immunohistochemistry analysis of RBM17 in paraffin-embedded human endometrium cancer. Samples were incubated with RBM17 Polyclonal antibody (Product # PA5-91967) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of RBM17 in paraffin-embedded human endometrium cancer using RBM17 Polyclonal Antibody (Product # PA5-91967) at a dilution of 1:100.
Western blot analysis of RBM17 in extracts of various cell lines. Samples were incubated with RBM17 Polyclonal antibody (Product # PA5-91967) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Supplier Page from Thermo Fisher Scientific for RBM17 Antibody