Western Blot using KChIP3 Polyclonal Antibody (Product # PA5-80750) at 1:500 dilution. Lane A: Mouse heart Tissue Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 28 kDa. Observed band size: 36 kDa.

KChIP3 Immunoprecipitation using: Lane A: 0.5 mg MCF-7 Whole Cell Lysate, Lane B: 0.5 mg THP-1 Whole Cell Lysate 0.5 µL with KChIP3 Polyclonal Antibody (Product # PA5-80750) and 15 µL of 50 % Protein G agarose. Primary antibody: KChIP3 Polyclonal Antibody, at 1:350 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 28 kDa. Observed band size: 35 kDa.