Western Blot using E-selectin Recombinant Rabbit Monoclonal Antibody (15) (Product # MA5-29785) at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate, Lane B: Mouse liver tissue lysate, Lane C: Mouse spleen tissue lysate, Lane D: Mouse brain tissue lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 67 kDa. Observed band size: 67 kDa.
Supplier Page from Thermo Fisher Scientific for E-selectin Antibody