Western Blot analysis of CD32b (FCGR2B) in extracts of various cell lines using CD32b Polyclonal Antibody (Product # PA5-88621) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Western blot analysis of CD32b in extracts of various cell lines. Samples were incubated with CD32b Polyclonal antibody (Product # PA5-88621) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.