Immunocytochemistry analysis of PTHLH in 4% paraformaldehyde-fixed HepG2 cells using PTHLH polyclonal antibody (Product # PA5-85106) at a dilution of 1:500. Sample was then incubated with Hoechst secondary antibody.

Western blot analysis of PTHLH in A) HepG2, B) HCT116 cells using PTHLH polyclonal antibody (Product # PA5-85106) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 12% SDS PAGE.