Immunofluorescence staining of GCNF in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with GCNF Polyclonal Antibody (Product # PA5-81232, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to nucleus.

Western Blot using GCNF Polyclonal Antibody (Product # PA5-81232) at 1:500 dilution. Lane A: HEK293 Whole Cell Lysate, Lane B: MCF7 Whole Cell Lysate, Lane C: K562 Whole Cell Lysate, Lane D: Hela Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 54 kDa. Observed band size: 60 kDa. (We are unsure of the identity of these extra bands).