Western Blot using MAX Polyclonal Antibody (Product # PA5-80800) at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 23 kDa.

MAX Immunoprecipitation using: Lane A: 0.5 mg Jurkat Whole Cell Lysate, Lane B: 0.5 mg Hela Whole Cell Lysate, Lane C: 0.5 mg 293T Whole Cell Lysate, Lane D: 0.5 mg A549 Whole Cell Lysate 2 µL with MAX Polyclonal Antibody (Product # PA5-80800) and 15 µL of 50 % Protein G agarose. Primary antibody: MAX Polyclonal Antibody, at 1:330 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 23 kDa. Observed band size: 23 kDa.