Immunohistochemistry analysis of CDK5RAP1 in paraffin-embedded human colon carcinoma. Samples were incubated with CDK5RAP1 Polyclonal antibody (Product # PA5-90755) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Western blot analysis of CDK5RAP1 in extracts of various cell lines. Samples were incubated with CDK5RAP1 Polyclonal antibody (Product # PA5-90755) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
Immunocytochemistry-Immunofluorescence analysis of CDK5RAP1 was performed in L-929 cells using CDK5RAP1 Polyclonal Antibody (Product # PA5-90755).
Supplier Page from Thermo Fisher Scientific for CDK5RAP1 Antibody