For immunofluorescence analysis, PANC-1 cells were fixed and permeabilized for detection of endogenous PKM2 using Anti-PKM2 Recombinant Rabbit Monoclonal Antibody (Product # 703707, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962) and cytoskeletal F-actin (red) staining using Rhodamine Phalloidin (Product # R415, 1:300) Panel a-d) shows representative un-treated cells that were stained for detection and localization of PKM2 protein (green) with enhanced signal in cytoplasm compared to panel e-h) clearly demonstrating reduction in cytoplasmic localisation of PKM2 in glucose-deprived (72h) PANC-1 cells. The images were captured at 60X magnification.