Immunocytochemistry-Immunofluorescence analysis of MAP2 was performed in DIV9 rat E18 primary cortical neurons fixed in 4% paraformaldehyde at RT for 15 min. Green: MAP2 Polyclonal Antibody (Product # PA5-85756) diluted at 1:500. Red (Top right): Dendrites, stained by MAP2 antibody. Red (Bottom right): Axons, stained by Tau antibody diluted at 1:1000. Blue: Fluoroshield with DAPI.

MAP2 Polyclonal Antibody detects MAP2 protein in dendrites, but not in axons, by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MAP2 stained by MAP2 Polyclonal Antibody (Product # PA5-85756) diluted at 1:500. Red: Tau, stained by Phospho-Tau (Ser262) Polyclonal Antibody [GT287]diluted at 1:500. Blue: Fluoroshield with DAPI .