Immunofluorescence staining of Human CD55 in JURKAT cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with CD55 Recombinant Rabbit Monoclonal Antibody (28) (Product # MA5-29118, 1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.

Flow cytometric analysis of human CD55 expression on K562 cells. Cells were stained with CD55 Recombinant Rabbit Monoclonal Antibody (28) (Product # MA5-29118), then a FITC-conjugated Secondary antibody. The histograms were derived from events with the forward and side light-scatter characteristics of intact cells.