Western Blot using WNT5A Polyclonal Antibody (Product # PA5-85302). Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with WNT5A Polyclonal Antibody (Product # PA5-85302) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Western blot analysis of WNT5A in A) non-transfected 293T lysate, B) WNT5A transfected 293T lysate using WNT5A polyclonal antibody (Product # PA5-85302) using 30 µg of sample at a dilution of 1:5000. Prior to incubation with primary antibody, the sample was separated on 10% SDS PAGE.