Confocal image of human lymph node imaged using IBEX . CD4+ T cells and macrophages were labeled with CD4 Monoclonal Antibody (RPA-T4), Alexa Fluor™ 532, eBioscience™ (Product # 58-0049-42, 1:25 dilution) (cyan). CD8 T cells (red) were detected with a monoclonal antibody (clone SK1). Tissues were prepared using ~1% (vol/vol) formaldehyde and a detergent. Following fixation, samples are immersed in 30% (wt/vol) sucrose for cryoprotection. Images are courtesy of Drs. Andrea J. Radtke and Ronald N. Germain of the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH). See for additional community-validated antibodies compatible with IBEX.

Normal human peripheral blood cells were stained with CD3 Monoclonal Antibody, APC (Product # 17-0038-42) and Mouse IgG1 kappa Isotype Control, Alexa Fluor 532 (Product # 58-4714-80) (left) or CD4 Monoclonal Antibody, Alexa Fluor 532 (right). Cells in the lymphocyte gate were used for analysis.