Western blot was performed using Anti-Granzyme B Recombinant Rabbit Monoclonal Antibody (2) (Product # MA5-29315) and a 32 kDa band corresponding to Granzyme B was observed only in NK-92 cells which is reported to be positive and not in any other cell lines. Whole cell extracts (30 µg lysate) of NK-92 (Lane 1), KARPAS 299 (Lane 2), Jurkat (Lane 3), K-562 (Lane 4), Daudi (Lane 5), RAW 264.7 (Lane 6), BeWo (Lane 7) and SH-SY5Y (Lane 8) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).