Immunofluorescence analysis of K-Ras in NIH/3T3 cells. Samples were incubated with K-Ras Polyclonal antibody (Product # PA5-88269) using a dilution of 1:100. Blue: DAPI for nuclear staining.
Immunocytochemistry-Immunofluorescence analysis of K-Ras was performed in C6 cells using K-Ras Polyclonal Antibody (Product # PA5-88269) at a dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of K-Ras in extracts of various cell lines. Samples were incubated with K-Ras Polyclonal antibody (Product # PA5-88269) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Supplier Page from Thermo Fisher Scientific for K-Ras Antibody