Western blot was performed using CD98 Recombinant Rabbit Monoclonal Antibody (Product # MA5-29814) and a 80 kDa band corresponding to CD98 was observed across tissue extracts except Mouse, Rat Lung and Mouse, Rat Skeletal Muscle, along with an uncharacterized band (*) at ~60 kDa in the tissue extracts tested. Tissue extracts (30 µg lysate) of Mouse Kidney (Lane 1), Mouse Testis (Lane 2), Mouse Lung (Lane 3), Mouse Thymus (Lane 4), Mouse Skeletal Muscle (Lane 5), Rat Kidney (Lane 6), Rat Testis (Lane 7), Rat Lung (Lane 8) and Rat Skeletal Muscle (Lane 9) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (1:5,000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4,000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).