Western blot was performed using Anti-PLZF Polyclonal Antibody (Product # PA5-81949) and a 74 kDa band corresponding to ZBTB16 was observed across positive cell lines LNCaP, HEL 92.1.7 and mouse tissue lysates but not in negative cell lines HL-60 and SK-OV-3. Tissue lysates also detect the second isoform of PLZF (~61 kDa). Nuclear enriched extracts (30 µg lysate) of LNCaP (Lane 1), HEL 92.1.7 (Lane 2), HL-60 (Lane 3), SK-O-V3 (Lane 4), Mouse Heart (Lane 5), Mouse Ovary (Lane 6) and Mouse Thymus (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.3 µg/mL dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:10000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580). An uncharacterized band (*) was observed at ~40 kDa across cell and tissue lysates except in HL-60 and Mouse Heart. Additionally, the tissue lysates display two other uncharacterized bands (*) at ~55 kDa and ~80 kDa.