Western Blot using STAT4 Polyclonal Antibody (Product # PA5-81044) at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate, Lane B: K562 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 86 kDa. Observed band size: 86 kDa. (We are unsure of the identity of these extra bands).

Immunofluorescence staining of STAT4 in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with STAT4 Polyclonal Antibody (Product # PA5-81044, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to cytoplasm and nucleus..