C57Bl/6 mouse splenocytes were stained with Anti-Mouse CD4 APC (Product # 17-0041-82) and staining buffer (autofluorescence, left) or 0.125 µg of Rat IgG1 kappa Isotype Control, eFluor 506 (right). Total viable cells were used for analysis.
Supplier Page from Thermo Fisher Scientific for Rat IgG1 kappa eFluor 506 Isotype Control