Western Blot using NT5C3A Recombinant Rabbit Monoclonal Antibody (9) (Product # MA5-29453) at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 38 kDa. Observed band size: 39 kDa.

NT5C3A Immunoprecipitation using: Lane A: 0.5 mg 293T Whole Cell Lysate, Lane B: 0.5 mg Jurkat Whole Cell Lysate 0.5 µL with NT5C3A Recombinant Rabbit Monoclonal Antibody (9) (Product # MA5-29453) and 15 µL of 50 % Protein G agarose. Primary antibody: NT5C3A Recombinant Rabbit Monoclonal Antibody (9), at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 38 kDa. Observed band size: 38 kDa.