Immunofluorescence analysis of CHK2 in HeLa cells. Samples were incubated with CHK2 Polyclonal antibody (Product # PA5-87441). Blue: DAPI for nuclear staining.
Immunocytochemistry-Immunofluorescence analysis of CHK2 was performed in A549 cells using CHK2 Polyclonal Antibody (Product # PA5-87441).
Western blot analysis of CHK2 in extracts of various cell lines. Samples were incubated with CHK2 Polyclonal antibody (Product # PA5-87441) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Supplier Page from Thermo Fisher Scientific for CHK2 Antibody