SPARC Immunoprecipitation using: Lane A: 0.5 mg A549 Whole Cell Lysate, Lane B: 0.5 mg 293T Whole Cell Lysate 0.5 µL with SPARC Recombinant Rabbit Monoclonal Antibody (1) (Product # MA5-29821) and 15 µL of 50 % Protein G agarose. Primary antibody: SPARC Recombinant Rabbit Monoclonal Antibody (1), at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 35 kDa. Observed band size: 38 kDa.

Western Blot using SPARC Recombinant Rabbit Monoclonal Antibody (1) (Product # MA5-29821) at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 35 kDa. Observed band size: 40 kDa.