Immunocytochemistry-Immunofluorescence analysis of LRRC7 was performed in U-2 OS cells using LRRC7 Polyclonal Antibody (Product # PA5-88994) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of LRRC7 in extracts of various cell lines. Samples were incubated with LRRC7 Polyclonal antibody (Product # PA5-88994) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.