Immunocytochemistry-Immunofluorescence analysis of MYEF2 was performed in C6 cells using MYEF2 Polyclonal Antibody (Product # PA5-90739) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of MYEF2 in extracts of various cell lines. Samples were incubated with MYEF2 Polyclonal antibody (Product # PA5-90739) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.