Immunofluorescence staining of PRDX2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with PRDX2 Polyclonal Antibody (Product # PA5-80932, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

Western Blot using PRDX2 Polyclonal Antibody (Product # PA5-80932) at 1:500 dilution. Lane A: SH-SYSY Whole Cell Lysate, Lane B: HepG2 Whole Cell Lysate, Lane C: Jurkat Whole Cell Lysate, Lane D: MCF7 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti- RabbitIgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 22 kDa. Observed band size: 22 kDa. (We are unsure of the identity of these extra bands).