Western blot analysis of SNCA was performed by loading 30 µg of HeLa wildtype (Lane 1), HeLa SNCA knockout (Lane 2) whole cell extracts. The blot was probed with Anti-alpha Synuclein Recombinant Rabbit Monoclonal Antibody (17)(Product # MA5-29575) using 1:500 dilution and Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036) using 1:4000 dilution. Loss of signal upon CRISPR mediated knockout (KO) confirms that antibody is specific to SNCA. An uncharacterized band was observed at ~50 kDa.