IGFBP7 Immunoprecipitation using: Lane A: 0.5 mg 293T Whole Cell Lysate 0.5 µL with IGFBP7 Recombinant Rabbit Monoclonal Antibody (65) (Product # MA5-29345) and 15 µL of 50 % Protein G agarose. Primary antibody: IGFBP7 Recombinant Rabbit Monoclonal Antibody (65), at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 35 kDa. Observed band size: 35 kDa.