Western Blot using CD3d Recombinant Rabbit Monoclonal Antibody (6) (Product # MA5-29107) at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 19 kDa. Observed band size: 25 kDa. (We are unsure of the identity of these extra bands).

CD3d Immunoprecipitation using: Lane A: 0.5 mg HuT78 Whole Cell Lysate 0.5 µL with CD3d Recombinant Rabbit Monoclonal Antibody (6) (Product # MA5-29107) and 15 µL of 50 % Protein G agarose. Primary antibody: CD3d Recombinant Rabbit Monoclonal Antibody (6), at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 20 kDa. Observed band size: 20 kDa.