CD200 Antibody AF2724 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemCD200 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF2724
NCBI Gene AliasesOX-2, MOX1, MOX2, MRC
ApplicationsIF/IHC,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Simple Western,Immunocytochemistry,CyTOF-ready
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityHuman,Mouse,Rat,Transgenic Mouse
Quantity100 ug (also 25 ug)
TargetCD200
HostGoat
IsotypeIgG
NCBI Full Gene NameCD200 molecule
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant human CD200, Gln31-Gly232, Accession # P41217
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human CD200 in direct ELISAs and Western blots.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Increased soluble CD200 in serum of SLE patients does not reflect disease activity or affect CD200R1 function in T cellsArthritis Research & TherapyMarch 16, 2026Ellen D. Kaan, Fabienne Verburg, Maarten Limper, Linde Meyaard, Michiel van der Vlistusing monoclonal anti-CD200 (Sino Biological #10,886-R005) as capture and polyclonal CD200 (Bio-Techne #AF2724) as detection antibody. The protocol and assay validation are enclosed as Supplementary document 1.

(2) A comprehensive molecular atlas of the mesenchymal cell types in the mouse liverEMBO ReportsSeptember 15, 2025Riikka Pietilä, Guillem Genové, Giuseppe Mocci, Yuyang Miao, Jianping Liu, Stefanos Leptidis, Francesca Del Gaudio, Martin Uhrbom, Elisa Vázquez-Liébanas, Sonja Gustafsson, et al.,R&D Systems,AF3437 Alpha-SMA-Cy3 (mouse),Sigma,C6198 Alpha-SMA-FITC (mouse),Sigma,F3777 CD200 (goat),R&D Systems,AF2724 CD31 (rat),BD Bioscience,550274 CD31 (goat),R&D Systems,AF3628 CD31-APC (rat),BD Bioscience,561814 CD31-FITC

(3) Pathology-oriented multiplexing enables integrative disease mappingNatureAugust 1, 2025Kuehl M, Okabayashi Y, Wong MN, Gernhold L, Gut G, Kaiser N, Schwerk M, Gräfe SK, Ma FY, Tanevski J, et al.

(4) Msx1(+) stem cells recruited by bioactive tissue engineering graft for bone regenerationNature communicationsSeptember 5, 2022Zhang X, Jiang W, Xie C, Wu X, Ren Q, Wang F, Shen X, Hong Y, Wu H, Liao Y, et al.Invitrogen, USA); CD105-PE (85-12-1057-42, eBioscience, San Diego, CA, USA); and CD200 (0.25 μg/106 cells, AF2724, R&D Systems, USA); Msx1 (diluted 200×, NBP2-57969, NOVUS, USA).) for 1 h on ice. The cells were then

(5) Oligodendrocyte-Specific Deletion of FGFR1 Reduces Cerebellar Inflammation and Neurodegeneration in MOG(35-55)-Induced EAEInternational journal of molecular sciencesAugust 31, 2021Rajendran R, Rajendran V, Giraldo-Velasquez M, Megalofonou FF, Gurski F, Stadelmann C, Karnati S, Berghoff M.Tech, MA, USA" Anti-SEMA3A,Rabbit,89 kDa,WB,Ab23393,"Abcam, Cambridge, UK" Anti-CD200,Goat,42 kDa,WB,AF2724,"R&D Systems, MN, USA" Anti-pP38,Rabbit,43 kDa,WB,9215,"Cell Signaling Tech, MA, USA" Anti-pJNK,Rabbit,"46,

(6) Defining the lineage of thermogenic perivascular adipose tissueNature metabolismApril 1, 2021Angueira AR, Sakers AP, Holman CD, Cheng L, Arbocco MN, Shamsi F, Lynes MD, Shrestha R, Okada C, Batmanov K, et al.600–401-379, RRID:AB_2209751), GFP (1:500, Abcam: AB6673, RRID:AB_305643), CD200: (1:25, Novus Biologicals: AF2724, RRID:AB_416669), ACTA2: Sigma: (1:200, A2547, RRID:AB_476701), TAGLN: (1:100, Abcam: ab10135, RRID:AB_2255631),

(7) Distinct immune regulatory receptor profiles linked to altered monocyte subsets in sarcoidosisERJ open researchJanuary 1, 2021Fraser SD, Crooks MG, Kaye PM, Hart SP.and CD200R was adapted from Cawkwell et al. [9]. Antibodies were a polyclonal goat CD200L antibody (AF2724; R&D Systems, Abingdon, UK), normal goat IgG control (AB-108-C; R&D Systems), mouse monoclonal IgG1

(8) An optimized quantitative proteomics method establishes the cell type-resolved mouse brain secretomeThe EMBO journalOctober 15, 2020Tüshaus J, Müller SA, Kataka ES, Zaucha J, Sebastian Monasor L, Su M, Güner G, Jocher G, Tahirovic S, Frishman D, et al.#75‐093), rat monoclonal anti‐SEZ6 (Pigoni et al, 2016), goat polyclonal anti‐CD200 (R and D Systems Cat #AF2724), and rabbit polyclonal anti‐calnexin (Enzo Life Sciences Cat #ADI‐SPA‐860). ELISA CD200 ectodomain

(9) Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizerNature communicationsOctober 14, 2020Apóstolo N, Smukowski SN, Vanderlinden J, Condomitti G, Rybakin V, Ten Bos J, Trobiani L, Portegies S, Vennekens KM, Gounko NV, et al.MAB1624;RRID: AB_2207001 Sheep anti-Contactin 1,R&D Systems,Cat#: AF7549 Goat anti-CD200,R&D Systems,Cat#: AF2724 Goat anti-ICAM5,Novus,Cat#: NB100-53815;RRID: AB_829163 Goat anti-SCFR/Kit,R&D Systems,Cat#: AF1356;RRID:

(10) Transcriptome analysis of desmoplastic small round cell tumors identifies actionable therapeutic targets: a report from the Children's Oncology GroupScientific reportsJuly 23, 2020Hingorani P, Dinu V, Zhang X, Lei H, Shern JF, Park J, Steel J, Rauf F, Parham D, Gastier-Foster J, et al.Santa Cruz), FGFR4 (#8562; Cell Signaling), GAPDH (#5174; Cell Signaling), IGF2 (ab9574; Abcam), CD200 (AF2724; R&D Systems), or B7-H3 (#14058; Cell Signaling). The membranes were incubated with HRP conjugated secondary

(11) Reduced CD200 expression is associated with altered Th1/Th2 cytokine production in placental trophoblasts from preeclampsiaAm J Reprod ImmunolJanuary 1, 2018Xu J, Gu Y, Sun J, Zhu H, Lewis DF, Wang Y.cytokine production in preeclampsia. Materials and Methods Chemicals and reagents Antibody for CD200 (AF2724) and DuoSet ELISA kits for human TNFα (DY210), soluble TNFR1 (sTNFR1, DY225), INFγ (DY285), IL-4 (DY204),

(12) Changes in CD200 and intercellular adhesion molecule-1 (ICAM-1) levels in brains of Lewy body disorder cases are associated with amounts of Alzheimer's pathology not α-synuclein pathologyNeurobiology of agingJune 1, 2017Walker DG, Lue LF, Tang TM, Adler CH, Caviness JN, Sabbagh MN, Serrano GE, Sue LI, Beach TG.

(13) CD200-positive cancer associated fibroblasts augment the sensitivity of Epidermal Growth Factor Receptor mutation-positive lung adenocarcinomas to EGFR Tyrosine kinase inhibitorsScientific reportsApril 21, 2017Ishibashi M, Neri S, Hashimoto H, Miyashita T, Yoshida T, Nakamura Y, Udagawa H, Kirita K, Matsumoto S, Umemura S, et al.

(14) Immunoprofiling of Adult-Derived Human Liver Stem/Progenitor Cells: Impact of Hepatogenic Differentiation and InflammationStem cells internationalJanuary 1, 2017El-Kehdy H, Sargiacomo C, Fayyad-Kazan M, Fayyad-Kazan H, Lombard C, Lagneaux L, Sokal E, Najar M, Najimi M.serum albumin (BSA). Cells were incubated for 1 hour with polyclonal goat anti-CD200 primary antibody (AF2724 R&D systems) at room temperature. After washing with PBS, ADHLSCs were incubated for 30 min with donkey

(15) Transcriptomic Analysis of Cultured Corneal Endothelial Cells as a Validation for Their Use in Cell Replacement TherapyCell transplantationJanuary 1, 2016Frausto RF, Le DJ, Aldave AJ.IgG,human,polyclonal,1:1000,Cell Signaling Technology,3010 CD200,Goat IgG,human,polyclonal,1:1000,R&D Systems,AF2724 CDH2,rabbit IgG,human,polyclonal,1:1000,Cell Signaling Technology,4061 COL8A2,rabbit IgG,human,polyclonal,1:1000,Santa Cruz Biotechnology

(16) CD200: association with cancer stem cell features and response to chemoradiation in head and neck squamous cell carcinomaHead & neckMarch 1, 2015Jung YS, Vermeer PD, Vermeer DW, Lee SJ, Goh AR, Ahn HJ, Lee JH.calf serum and 1% penicillin/streptomycin. Antibodies Anti-CD200 (R & D Systems, Minneapolis, MN, AF2724), anti-Shh (Santa Cruz Biotechnology, Santa Cruz, CA, sc-9024), anti-Bmi-1 (clone F6, Millipore Corp.,

(17) Utility of CD200 immunostaining in the diagnosis of primary mediastinal large B cell lymphoma: comparison with MAL, CD23, and other markersModern pathology : an official journal of the United States and Canadian Academy of Pathology, IncDecember 1, 2012Dorfman DM, Shahsafaei A, Alonso MA.

(18) Cell-surface markers for the isolation of pancreatic cell types derived from human embryonic stem cellsNature biotechnologyJuly 31, 2011Kelly OG, Chan MY, Martinson LA, Kadoya K, Ostertag TM, Ross KG, Richardson M, Carpenter MK, D'Amour KA, Kroon E, et al.

(19) Bald scalp in men with androgenetic alopecia retains hair follicle stem cells but lacks CD200-rich and CD34-positive hair follicle progenitor cellsThe Journal of clinical investigationFebruary 1, 2011Garza LA, Yang CC, Zhao T, Blatt HB, Lee M, He H, Stanton DC, Carrasco L, Spiegel JH, Tobias JW, et al.

(20) CD200 is induced by ERK and is a potential therapeutic target in melanomaThe Journal of clinical investigationDecember 1, 2007Petermann KB, Rozenberg GI, Zedek D, Groben P, McKinnon K, Buehler C, Kim WY, Shields JM, Penland S, Bear JE, et al.

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CD200 Antibody

CD200 Antibody

CD200 Antibody

CD200 Antibody

CD200 Antibody

CD200 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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CD200 Antibody from R&D Systems, a Bio-Techne Brand

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