Progranulin/PGRN Antibody AF2557 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemProgranulin/PGRN Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $529.00Supplier Page View Company Product Page
Catalog NumberAF2557
NCBI Gene AliasesPEPI, CLN11, GEP, PGRN, PCDGF, epithelin, GP88, Pgrn
ApplicationsELISA Development (Detection),Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,In vivo assay,Immunofluorescence,Immunocytochemistry,ELISA Capture
Conjugate/TagUnconjugated
ReactivityHuman,Mouse,Rat,Transgenic Mouse
Quantity100 ug (also 25 ug)
IsotypeIgG
TargetProgranulin/PGRN
HostSheep
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
NCBI Full Gene Namegranulin, granulin precursor
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse Progranulin, Thr18-Leu589, Accession # P28798
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse Progranulin in direct ELISAs and Western blots. In Western blots, approximately 15% cross-reactivity with recombinant human Progranulin is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

What applications has the Progranulin/PGRN antibody been used for in scientific research?

The Progranulin/PGRN antibody has been shown to work in various applications including Western blotting, ELISA, immunocytochemistry, immunofluorescence, and immunohistochemistry, as evidenced by its use in multiple studies to detect mouse Progranulin in these contexts.

How does the Progranulin/PGRN antibody perform in terms of specificity and cross-reactivity?

This antibody specifically detects mouse Progranulin in direct ELISAs and Western blots, with approximately 15% cross-reactivity observed with recombinant human Progranulin, making it suitable for studies involving mouse models while allowing for some comparative analysis with human samples.

Are there any notable findings from studies that utilized the Progranulin/PGRN antibody?

Yes, studies utilizing this antibody have demonstrated that progranulin plays a protective role in neurodegenerative conditions. For instance, in a study on spinal cord injury, PGRN deficiency was shown to exacerbate inflammation and apoptosis, highlighting the antibody's utility in understanding the role of PGRN in neuroinflammation and neurodegeneration.

What is the recommended dilution for using the Progranulin/PGRN antibody in Western blotting?

The Progranulin/PGRN antibody is typically used at a dilution of 1:500 for Western blot applications, as indicated in various research articles that have successfully employed this antibody for protein detection.

R&D Systems, a Bio-Techne Brand

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Company Profile

Website: http://www.rndsystems.com

(1) Progranulin deficiency in the brain activates an insulin signaling pathway that may promote neurodegenerationiScienceMay 15, 2026Mini P. Sajan, Geetika Aggarwal, Joel Jihwan Hwang, Denise M. Smith, Denise R. Cooper, Mildred Acevedo Duncan, Barbara C. Hansen, Andrew D. Nguyen, Robert V. Fareseof human progranulin (generated as described in Nguyen et al.127), mouse progranulin (R&D Systems, AF2557), β-Actin (Cell Signaling, 8457), Vinculin (Cell Signaling, 13901), JNK (Cell Signaling, 9252), IRS-1

(2) Progranulin-driven lysosomal acidification facilitates exocytosis of PHEV-hijacked lysosomes for viral releasemBioJanuary 14, 2026Zhenzhen Wang, Yuzhu Chen, Wenqi He, Yuzhu Liu, Yubo Jiao, Gaili Wang, Jiyu Guan, Kui Zhao, Qiaoling Zhang, Feng Gao, et al.(ab188586), anti-ATP6V1A (ab199326), and anti-ATP6V1B1 + ATP6V1B2 (ab200839) from Abcam; anti-PGRN (AF2557) from R&D Systems; anti-CD107a (553792) from BD Biosciences; anti-Arl8b (13049-1-AP), anti-ATP6V0a3

(3) Ablation of progranulin augments microglial activation and accelerates prion progressionActa Neuropathol CommunOctober 13, 2025Bei Li, Yiyue Shi, Wenyu Hou, Haoyuan Guan, Jun Li, Tuo Yi, Wei Li, Donglin Cai, Petra Schwarz, Adriano Aguzzi, et al.LAS-3000 system. Primary antibodies used in this study included anti-PGRN antibody (1:400, R&D system, AF2557), POM1 (200 ng ml- 1), anti-Iba1 (1:1,000, Wako laboratory chemicals, 019-19741), anti-GFAP (1:2,000,

(4) Progranulin deficiency does not exacerbate TDP-43 pathology in TDP-43 transgenic mouse modelsNPJ DementJuly 21, 2025Cha Yang, Tuancheng Feng, Fenghua Hu(Millipore, SMI-99), rabbit anti-MAG (Proteintech Group, 14386-1-AP), sheep anti-mouse PGRN (R&D systems, AF2557), and mouse anti-GAPDH (Proteintech Group, 60004-1-Ig). Mouse strains Grn−/− mice48 and TDP-43Q331K

(5) Loss of FMRP in microglia promotes degeneration of parvalbumin neurons and audiogenic seizures via progranulin insufficiencybioRxiv : the preprint server for biologyJuly 12, 2025Li H, Choi JJ, Huang EJ, Xu B.(abcam, ab17722, 1:1000), mouse anti-α-Tub (Sigma, T6074, 1:10000), sheep anti-progranulin (R&D system, AF2557, 1:1000), rabbit anti-GAPDH (Cell signaling, 2118, 1:3000), rabbit anti-CTSD (Proteintech, 21327–1,

(6) sPLA2-IIA modifies progranulin deficiency phenotypes in mouse modelsMol NeurodegenerJune 17, 2025Cha Yang, Huan Du, Gwang Bin Lee, Masaaki Uematsu, Weiguo He, Etienne Doré, Weizhi Yu, Ethan J. Sanford, Marcus B. Smolka, Eric Boilard, et al.LAMP1 (1D4B) (BD Biosciences, 553793, 1:300 for immunostaining), sheep anti-mouse PGRN (R&D systems, AF2557, 1:1000 for western blot and 1:100 for immunostaining), goat anti-CathD (R&D systems, AF1029, 1:1000

(7) Macrophage-Specific Progranulin Deficiency Prevents Diet-Induced Obesity through the Inhibition of Hypothalamic and Adipose Tissue InflammationDiabetes & Metabolism JournalMarch 11, 2025Chan Hee Lee, Chae Beom Park, Hyun-Kyong Kim, Won Hee Jang, Se Hee Min, Jae Bum Kim, Min-Seon Kimserum-containing PBS. The cells were then incubated with PGRN antibody (1:200, R&D Systems, Minneapolis, MN, USA, #AF2557) for 30 minutes followed by fluorescein Isothiocyanate (FITC)-conjugated anti-sheep secondary antibody

(8) Progranulin deficiency attenuates tubulointerstitial injury in a mouse unilateral ureteral obstruction modelExperimental AnimalsJuly 9, 2024Eri Adachi, Maki Murakoshi, Terumi Shibata, Kenta Shimozawa, Hiroko Sakuma, Chiaki Kishida, Tomohito Gohda, Yusuke Suzukiwere then performed using polyclonal sheep anti-PGRN antibodies (1:20 dilution in 1% bovine serum, AF2557; R&D Systems, Minneapolis, MN, USA) overnight at 4°C. Next, the sections were incubated with Alexa Fluor

(9) Reduced progranulin increases tau and α-synuclein inclusions and alters mouse tauopathy phenotypes via glucocerebrosidaseNature communicationsFebruary 16, 2024Takahashi H, Bhagwagar S, Nies SH, Ye H, Han X, Chiasseu MT, Wang G, Mackenzie IR, Strittmatter SM.

(10) Progranulin protects against Clostridioides difficile infection by enhancing IL-22 productionGut microbesJanuary 1, 2024Huang J, Liu B, Liu Y, You W, Zhao P, Liu Y, Wang K, Lai X, Xu B, Cao J.difficile.35 For PGRN neutralization, WT mice were administrated with 100 μg anti-PGRN (R&D Systems, AF2557) or IgG isotype control (R&D Systems, MAB006) on days −1, 1 and 2 of C. difficile infection. DNA extraction

(11) Dysregulation of the progranulin-driven autophagy-lysosomal pathway mediates secretion of the nuclear protein TDP-43Journal of Biological ChemistryNovember 1, 2023Yoshinori Tanaka, Shun-ichi Ito, Yuki Honma, Masato Hasegawa, Fuyuki Kametani, Genjiro Suzuki, Lina Kozuma, Kosuke Takeya, Masumi Eto610832, RRID: AB_398151) from BD Biosciences; a polyclonal sheep antimouse PGRN antibody (catalog no.: AF2557, RRID: AB_2114504) from R&D Systems; a monoclonal mouse anti-WIPI2 antibody (catalog no.: MCA5780GA,

(12) Prosaposin maintains lipid homeostasis in dopamine neurons and counteracts experimental parkinsonism in rodentsNature communicationsSeptember 19, 2023He Y, Kaya I, Shariatgorji R, Lundkvist J, Wahlberg LU, Nilsson A, Mamula D, Kehr J, Zareba-Paslawska J, Biverstål H, et al.

(13) AAV-GRN partially corrects motor deficits and ALS/FTLD-related pathology in Tmem106b(-/-)Grn(-/-) miceiScienceJuly 21, 2023Feng T, Minevich G, Liu P, Qin HX, Wozniak G, Pham J, Pham K, Korgaonkar A, Kurnellas M, Defranoux NA, et al.AB_2087094 goat anti-human PGRN,R&D Systems,Cat# AF2420; RRID: AB_2114489 sheep anti-mouse PGRN,R&D Systems,Cat# AF2557; RRID: AB_2114504 mouse anti-CHAT,R&D Systems,Cat# AF3447 mouse anti-NeuN,Millipore,Cat# MAB377; RRID:

(14) Loss of Progranulin Results in Increased Pan-Cathepsin Activity and Reduced LAMP1 Lysosomal ProteinbioRxiv : the preprint server for biologyJuly 16, 2023Anderson A, Tansey MG.

(15) Progranulin deficiency results in sex-dependent alterations in microglia in response to demyelinationActa neuropathologicaJuly 1, 2023Zhang T, Feng T, Wu K, Guo J, Nana AL, Yang G, Seeley WW, Hu F.(Novus Biologicals, NB100–1028), rat anti-CD68 (Bio-Rad, MCA1957), and sheep anti-PGRN (R&D Systems, AF2557), mouse anti-MBP (Millipore, SMI-99), Goat anti-Olig2 (R&D Systems, AF2418), mouse anti-APC (Millipore,

(16) Progranulin inhibits phospholipase sPLA2-IIA to control neuroinflammationbioRxiv : the preprint server for biologyApril 6, 2023Du H, Yang C, Nana AL, Seeley WW, Smolka M, Hu F.LAMP1 (1D4B) (BD Biosciences, 553793, 1:300 for immunostaining), sheep anti-mouse PGRN (R&D systems, AF2557, 1:750 for western blot), goat anti-CathD (R&D systems, AF1029, 1:100 for immunostaining), sheep anti-mouse

(17) Targeting nonsense-mediated RNA decay does not increase progranulin levels in the Grn R493X mouse model of frontotemporal dementiaPloS oneJanuary 1, 2023Smith DM, Niehoff ML, Ling K, Jafar-Nejad P, Rigo F, Farr SA, Wilkinson MF, Nguyen AD.antibodies used for immunoblot analysis include: an anti-mouse progranulin polyclonal antibody (R&D Systems, AF2557, 1:200 dilution) and an anti-α-tubulin monoclonal antibody (Sigma, T9026, 1:2000 dilution). The HRP-conjugated

(18) Decreased Prosaposin and Progranulin in the Cingulate Cortex Are Associated with Schizophrenia PathophysiologyInternational journal of molecular sciencesOctober 10, 2022He Y, Zhang X, Flais I, Svenningsson P.applied, including rabbit anti-PSAP (1:100, #10801-1-AP, Proteintech, IL, USA), sheep anti-PGRN (1:100, #AF2557, R&D systems, Min, USA), mouse anti-Cre recombinase (1:100, MAB3120, Merck, NJ, USA). The following

(19) Progranulin-derived granulin E and lysosome membrane protein CD68 interact to reciprocally regulate their protein homeostasisThe Journal of biological chemistrySeptember 1, 2022Santos MN, Paushter DH, Zhang T, Wu X, Feng T, Lou J, Du H, Becker SM, Fragoza R, Yu H, et al.MCA1957), rabbit anti-CD68 (Abcam; catalog no.: 125212), sheep antimouse PGRN (R&D Systems; catalog no.: AF2557), mouse anti-Myc (catalog no.: 9E10; Hybridoma Bank), mouse anti-GAPDH (Proteintech Group; catalog no.:

(20) Efficient progranulin exit from the ER requires its interaction with prosaposin, a Surf4 cargoThe Journal of cell biologyFebruary 7, 2022Devireddy S, Ferguson SM.Transduction Laboratories), goat anti-cathepsin D (AF1014; R&D Systems), sheep anti-mouse progranulin (AF2557; R&D Systems), and mouse anti-LAMP1 (1D4B; DSHB). Alexa Fluor–conjugated secondary antibodies used for

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