Histone H3 [Trimethyl Lys4] Antibody (RM340)

Chromatin Immunoprecipitation: Histone H3 Antibody (RM340) [NBP2-77403] - SNAP-ChIP TM/ qPCR using anti-H3K4me3 rabbit monoclonal antibody NBP2-77403. Antibody was tested in native ChIP with 3 ug HEK-293 chromatin (~1x106 cells). Specificity (left Y-axis; all bars mean SEM from six independent ChIP experiments; note <15% cross reactivity outside H3K4me3) was determined by quantitative real-time PCR (qPCR) for the duplicate DNA barcodes corresponding to each modified nucleosome in the SNAP-ChIP K-MetStat panel (X-axis). Black bar represents antibody efficiency (right Y-axis; log scale) and indicates percentage of the barcoded H3K4me3 nucleosome target immunoprecipitated relative to Input.

Chromatin Immunoprecipitation: Histone H3 Antibody (RM340) [NBP2-77403] - SNAP-ChIP TM/ qPCR using anti-H3K4me3 rabbit monoclonal antibody NBP2-77403. Antibody was tested in native ChIP with 3 ug HEK-293 chromatin (~1x106 cells). Specificity (left Y-axis; all bars mean SEM from six independent ChIP experiments; note <15% cross reactivity outside H3K4me3) was determined by quantitative real-time PCR (qPCR) for the duplicate DNA barcodes corresponding to each modified nucleosome in the SNAP-ChIP K-MetStat panel (X-axis). Black bar represents antibody efficiency (right Y-axis; log scale) and indicates percentage of the barcoded H3K4me3 nucleosome target immunoprecipitated relative to Input.