Fig 1: LYZ+ cells are Mislocalized in acute and germline tuft cell deletion models. (a) Immunostaining for LYZ (green) and ACTG1 (magenta) in jejunum. in control tissues, LYZ+ Paneth cells are limited to the bottom of crypts, whereas DCLK1‐DTA mice at Day 4 possess LYZ+ cells in upper crypt and villus regions (arrows). PAS staining was performed on the same slides after immunostaining. Insets show LYZ− goblet cells and LYZ+ Paneth cells in control and LYZ+ mislocalized cells in DCLK1‐DTA mice. Scale bars = 50 μm. (b) LYZ+ cells localized out of the crypt bottom are counted among 100 villi of jejunum (ANOVA with Tukey's test). (c) POU2F3―/― mouse tissues demonstrate mislocalized LYZ+ (green) cells out of crypts (arrow). (d) Co‐immunostaining for TFF3 (yellow), LYZ (magenta), and Zenon‐labeled MMP7 (cyan) in jejunum. ACTG1 and nuclei are shown in blue for visualizing tissue morphology. Triple‐positive cells for all markers (white arrows) are localized in villus region of DCLK1‐DTA day 4 tissues. (e, f) Digital image analysis for immunostained cell quantification in entire small intestine (two‐way ANOVA with LSD test). TFF3‐single positive cell number per all epithelial cells is significantly reduced in DCLK1‐DTA mouse small intestine (e). There was no difference in frequency of Paneth cell marker‐positive, TFF3− cells. Double‐ or triple‐positive cell numbers for both goblet and Paneth cell markers are shown as intermediate (IMED) type of cells (f).