Fig 2: P. berghei has a bimodal invasion pattern into either CD71-positive reticulocytes or normocytes.(A) UMAP of P. berghei cells colored by CD71 surface protein levels as measured by CITE-seq. (B) Proportion of CD71-positive cells per cluster in each sample. Organs are indicated by color [n = 6 (2 per organ), line at mean, Friedman test and Dunn’s post test]. (C) Host RNA levels, quantified as mouse-derived UMI (MmUMI), plotted against CD71 surface protein levels as measured by CITE-seq. Horizontal and vertical lines indicate thresholds separating CD71-positive (CD71 > 1.5) from CD71-negative cells and reticulocytes (MmUMI >100) from normocytes, respectively. (D) Host RNA levels across stages. Cells are randomly subsampled to 1000 cells per stage. Color of dots indicates CD71-positive (pink) and CD71-negative cells (black). (E) Average host RNA levels per cluster in each sample. Organs are indicated by color [n = 6 (2 per organ), line at mean, Friedman test and Dunn’s post test]. (F) Invasion of merozoites in CD71-positive reticulocytes (left) or CD71-normocytes (right) in the presence of anti-CD71 antibody (two different clones), anti-Ter119 antibody, or isotype control. Invasion was normalized to no antibody (AB) treatment and significance tested against isotype control (n = 6, means ± SEM, two-way ANOVA and Dunnett’s post test). (B, C, and E) *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.