Fig 1: HIF-1ß is required for viability of cells and Drosophila melanogaster following infection. (A) HeLa cells were transfected with control or HIF-1ß siRNA oligonucleotides. Where indicated, cells were treated with 100 ng/mL LIGHT for 4 or 24 h prior to whole cell lysis and Western blot analysis. Actin was used as a loading control. (B) HeLa cells were transfected as in 4A, but treated with 10 n/mL TNF-a for 4 h prior to lysis and Western blot analysis. Actin was used as a loading control. (C) Wild-type adult flies (w1118) and HIF-1ß (Tango) loss-of-function flies (Tango LF) were pricked using a thin needle dipped in a diluted overnight culture of Serratia marcescens Db10 (OD600 = 0.2) or in a saline solution (PBS, Mock). Groups of 100 flies were used and kept at room temperature. Survival was monitored and expressed as the ‘estimated probability of survival’. The p-value was obtained from log-rank statistical analysis: * p < 0.05. (D) Wild-type adult flies (w1118) and HIF-1ß (Tango) loss-of-function flies (Tango LF) were pricked using a thin needle dipped in a culture of Escherichia coli or in a saline solution (PBS, Mock). RNA was extracted 2 h later. Following cDNA synthesis, qPCR was performed using the indicated primers. Graphs depict mean and SEM from three independent biological experiments. Student t-test analysis was performed for each gene and levels of significance determined as: ns = not significant, * p < 0.05; *** p < 0.001.