Description
Product Characteristics: Decapping scavenger enzyme that catalyzes the cleavage of a residual cap structure following the degradation of mRNAs by the 3'->5' exosome-mediated mRNA decay pathway. Hydrolyzes cap analog structures like 7-methylguanosine nucleoside triphosphate (m7GpppG with up to 10 nucleotide substrates (small capped oligoribonucleotides and specifically releases 5'-phosphorylated RNA fragments and 7-methylguanosine monophosphate (m7GMP. Cleaves cap analog structures like tri-methyl guanosine nucleoside triphosphate (m3(2,2,7GpppG with very poor efficiency. Does not hydrolyze unmethylated cap analog (GpppG and shows no decapping activity on intact m7GpppG-capped mRNA molecules longer than 25 nucleotides. Does not hydrolyze 7-methylguanosine diphosphate (m7GDP to m7GMP. May also play a role in the 5'->3 mRNA decay pathway, m7GDP, the downstream product released by the 5'->3' mRNA mediated decapping activity, may be also converted by DCPS to m7GMP. Binds to m7GpppG and strongly to m7GDP. Plays a role in first intron splicing of pre-mRNAs. Inhibits activation-induced cell death.,DCPS,ARS,DCS1,HINT-5,HINT5,HSL1,HSPC015,Epigenetics & Nuclear Signaling,DCPS
Target Information: Necessary for the complete degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA fragments shorter than 10 nucleotides that are produced by 3'->5' exosome-mediated mRNA decay. Releases m7GMP. Can also degrade m7GDP to m7GMP. Has no activity towards mRNA molecules longer than 25 nucleotides