AMPKalpha (D5A2) Rabbit mAb 5831S from Cell Signaling Technology

Product Specs
ItemAMPKalpha (D5A2) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number5831S
Quantity100 µl
ApplicationsW, IP
ReactivityH, M, R, Mk, B
HostRabbit
ClonalityMonoclonal
TargetAMPK-alpha1;AMPK-alpha2
NCBI Full Gene Nameprotein kinase AMP-activated catalytic subunit alpha 2, protein kinase AMP-activated catalytic subunit alpha 1
NCBI Gene AliasesAMPK, AMPK alpha 1, PRKAA, AMPK2, AMPKa1, AMPKa2
Research AreaMetabolism
CloneD5A2
Molecular Weight62

Questions and Answers

Can this antibody be used to study AMPK in different species, and if so, which ones?

Yes, the AMPKalpha (D5A2) Rabbit mAb detects endogenous levels of AMPKα protein in multiple species, including humans, mice, rats, non-human primates, and bovines.

Is there any evidence of this antibody being used in studies related to inflammation or metabolic disorders?

Yes, the antibody has been utilized in research examining the role of AMPK in inflammation. For instance, in a study investigating colitis, AMPK activation was shown to inhibit pro-inflammatory cytokine secretion in macrophages, highlighting its importance in metabolic regulation during inflammatory responses ().

How has this antibody been applied in cancer research, particularly regarding cell death mechanisms?

The AMPKalpha (D5A2) Rabbit mAb has been used to explore the regulation of pyroptosis in tumor cells. Research indicates that AMPK negatively regulates the cytolytic activity of Gasdermin D, suggesting its role in modulating cell death pathways in cancer contexts ().

What applications has this antibody been shown to support in experimental setups?

The AMPKalpha (D5A2) Rabbit mAb has been shown to work effectively in Western blotting and immunoprecipitation applications, making it suitable for various experimental analyses of AMPK signaling pathways.

Cell Signaling Technology

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Phone: 978-867-2388
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Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Alpha-ketoglutarate alleviates acute pancreatitis by restoring energy metabolismiScienceApril 17, 2026Peng Li, Chunfeng Shi, Hangshi Wang, Shiyang Zhan, Jiahui Zhang, Dongyao Xu, Yuxun Lv, Furong Yan, Shaobin Xu, Jin Chen, et al.anti-p-AMPK (Thr172),Cell Signaling Technology,Cat# 2535S Rabbit anti-AMPK,Cell Signaling Technology,Cat# 5831T Rabbit anti-NDUFS3,Boster Biological Technology,Cat# A05867-2 Rabbit anti-SDHB,Boster Biological Technology,Cat#

(2) Saikosaponin D Is Associated with Anti-Tumor Effects and Markers of Autophagy and Endoplasmic Reticulum Stress in Human Endometrial Cancer Ishikawa CellsNutrientsApril 13, 2026Xiu-Xiu Zhang, Tong-Tong Tang, Xiao-Mei Ma, Kiran Thakur, Fei Hu, Jian-Guo Zhang, Yi-Long Ma, Zhao-Jun Weip-mTOR (Cell Signaling Technology, #5536, 1:1000, Danvers, MA, USA), AMPK (Cell Signaling Technology, #5831, 1:1000, Danvers, MA, USA), p-AMPK (Cell Signaling Technology, #2535, 1:1000, Danvers, MA, USA), and

(3) Polydatin Relieves Airway Remodeling by Inhibiting P2X7R-NLRP3-Mediated Excessive Autophagy in AsthmaImmun Inflamm DisApril 5, 2026Guangxing Li, Liangchang Li, Zhiguang Wang, Yihua Piao, Yilan Song, Li Li, Chang Xu, Xiaowan Li, Guanghai Yan(ab62557; Abcam), LKB1 (Ser428; #3482; CST), LKB1 (#3047; CST), AMPKα (Thr172; #2535; CST), AMPKα (#5831; CST), mTOR (Ser2448; #2971; CST), mTOR (#2983; CST), P2X7R (77665; Thermo), NLRP3 (MA523919; Lifescience),

(4) Apolipoprotein C3 exacerbates vascular calcification by promoting ferroptosis via the TLR2/AMPK pathway in vascular smooth muscle cellsRedox BiologyApril 1, 2026Zihao Zhou, Jinxuan Chen, Huan Wang, Yufei Han, Changyun Liu, Qiang Shen, Zhao Dong, Kaikai Lu, Chao Zhai, Yitong Xu, et al.summarized as follows: TLR2 (1:1000, ab209217, Abcam, USA), pAMPK (1:1000, 2535S, CST, USA), tAMPK (1:1000, 5831S, CST, USA), RUNX2 (1:1000, 12556S, CST, USA), NRF2 (1:1000, 12721S, CST, USA), ApoC3 (1:1000, YT5550,

(5) Nuclear Receptor Subfamily 4 Group A Member 2 (NR4A2) Induces a Warburg-like Effect and Promotes Phospholipids Synthesis in the Mouse HeartPhysiological GenomicsApril 1, 2026Sadia Ashraf, Dorcas Odogwu, David D. McPherson, Romain Harmanceyprotein 60 (HSP60; Cat no. 12165), the catalytic subunit of AMP-activated protein kinase (AMPKα; Cat. no. 5831) and phosphorylation of AMPKα at threonine 172 (Phospho-AMPKα (Thr172); Cat. no. 2535) were purchased

(6) AdipoR1–AMPK axis suppresses breast cancer across molecular subtypes via multimodal cell death pathways, including ferroptosis and apoptosisCell Death & DiseaseMarch 26, 2026Shinya Sato, Takashi Yamanaka, Yukako Komori, Mutsumi Ishida, Yoshiyasu Nakamura, Toshinari Yamashita, Yohei MiyagiCHOP, HO-1, BRCA1, BRCA2, ERα, and cleaved caspase 3 (Cell Signaling Technology, Danvers, MA, USA; 5831S, 2535S, 4970S, 3294S, 2895S, 43996S, 14823S, 10741S, 8644S, and 9664S, respectively). The blots were

(7) The Effect of Mechanical Loading on Mitophagy in Aged MyoblastsCellsMarch 15, 2026Evangelos Tolis, Eirini Chatzinikita, Athanasios Moustogiannis, Antonios Giannopoulos, Maria Maridaki, Michael Koutsilieris, Anastassios Philippou(PVDF) membrane, and were blotted with primary [AMPK Rabbit Ab (1:100; Cell Signaling Technology, #5831); p-Ulk1 (Ser 555) Rabbit Ab (1:1000; Cell Signaling Technology, #5869), BNIP3L/Nix Rabbit Ab (1:1000;

(8) Late‐life methionine restriction attenuates neuroinflammation in Alzheimer's disease mice via FGF21 activation in a metabolism‐independent mannerAlzheimer's & DementiaMarch 14, 2026Yuyu Zhang, Yiju Li, Qianxu Wang, Jingxi Pang, Da Wang, Tian Yuan, Zhigang Liuanti‐FGFR1 (CST #9740, 1:50), rabbit anti‐phospho‐AMPKα (CST #2535, 1:50), rabbit anti‐AMPKα (CST #5831, 1:50), and mouse anti‐β‐actin (Santa Cruz sc‐47778, 1:50)—were followed by HRP‐conjugated secondaries.

(9) Targeting mitochondrial bioenergetics: the “Achilles’ heel” of LeishmaniaParasites & VectorsMarch 9, 2026Deblina Sarkar, Sritama De Sarkar, Deep Goswami, Mitali ChatterjeeUSA); cell lysis buffer and antibodies against AMPKα (AMP-activated protein kinase-α; CST, cat. no. 5831); mammalian target of rapamycin (mTOR; CST, cat. no. 2983) or their phosphorylated counterpart P-AMPKα

(10) GLUD1 supports ovarian cancer progression by counteracting anoikis via ARAF/MEK/ERK signalingnpj Precision OncologyMarch 5, 2026Huolun Feng, Yanzhen Chen, Geyan Wu, Zhentao Zhang, Hongkun Lai, Changnian Yang, Shaofen Zhang, Yongqing Lin, Yingqi Liu, Haiyan Ye, et al.antibody, FAK(1:1000, Cat# 3285 T) antibody, p-FAK(1:1000, Cat# 8556 T) antibody, AMPK(1:1000, Cat# 5831 T) antibody, p-AMPK(1:1000, Cat# 2535 T) antibody, CREB(1:1000, Cat# 9197 T) antibody, p-CREB(1:1000,

(11) Mannose metabolic pathway senses glucose supply and regulates cell fate decisionsJournal of Biological ChemistryMarch 1, 2026Ziwei Wang, Yasuhide Miyamoto, Takehiro Suzuki, Miki Tanaka-Okamoto, Yu Mizote, Naoshi Dohmae, Hideaki Tahara, Naoyuki Taniguchi, Yoichiro Harada(Thr172) (40H9) (1:2000 dilution, 2535, Cell Signaling Technology), anti-AMPKα (D5A2) (1:5000 dilution, 5831, Cell Signaling Technology), anti-ATF4 (B-3) (1:1000 dilution, sc390063, Santa Cruz Biotechnology),

(12) Acceleration of Lactate Uptake and Utilization Contributes to Neuroprotective Action of FGF21 Involved in Naturally Aging MiceAging CellMarch 1, 2026Keru Ji, Hongde Wei, Ruguang Wang, Yutong Wu, Chen Li, Hongchang Gao, Liangcai Zhao(1:1000; 162901‐AP, Proteintech), p‐AMPK (1:1000; 2535S, Cell Signaling Technology), AMPK (1:1000; 5831S, Cell Signaling Technology), p‐Akt (1:1000; 4060S, Cell Signaling Technology), Akt (1:1000; 4691S, Cell Signaling Technology

(13) Targeting mitochondrial phosphatase PGAM5 alleviates ferroptosis and acute pancreatitis by upregulating NRF2-mediated FSP1 expressionCell Death & DiseaseFebruary 21, 2026Shuang Ma, Jianhua Qin, Jing Luan, Guoyan Hou, Jiyuan He, Minghui GaoCat#EP1808Y), anti-NRF2 (HUABIO, Cat#HA721432), anti-DRP1 (proteintech, Cat#12957-1-AP), anti-AMPKα (CST, Cat#5831), anti-Phospho-AMPKα (Thr172) (CST, Cat#2535), anti-FSP1 (proteintech, Cat#20886-1-AP), anti-Phospho-DRP1-Ser616

(14) VDAC1 protein derived from extracellular vesicles promotes paclitaxel resistance in gastric cancer through autophagy and mitophagyCancer Biology & MedicineFebruary 17, 2026Yanna Bi, Sisi Wei, Zhe Zhang, Yuqing Wang, Yang Wen, Hongquan Wang, Kexin Li, Cong Zhang, Yumin Wang, Lianmei Zhao, et al.(1:1000, 20986-1-AP; Proteintech); p-ULK1 (1:1000, #14202; Cell Signaling Technology); AMPK (1:1000, #5831; Cell Signaling Technology); p-AMPK (1:1000, #2535; Cell Signaling Technology); mTOR (1:5000, 20657-1-AP;

(15) Peiminine‐Induced Selective Autophagy of AIM2 Inflammasomes Rescues Cerebral Ischemic InjuryJournal of the American Heart AssociationFebruary 3, 2026Zhu‐chen Zhou, Meng‐ting Liu, Yi‐jia Fangma, Bing‐jie Wan, Jing Zhou, Xiao‐lan Zhang, Yu‐ting Yan, Shu‐hui Deng, Yan Zhang, Yuan‐jie Zhang, et al.(CST, 8054), Beclin (Abclone, A21191), p‐AMPK (p‐AMP‐activated protein kinase; CST, 2535), AMPK (CST, 5831), p‐Akt (p‐protein kinase B; CST, 4060), Akt (Abclone, A18120), Atg5 (Abclone, A19677), Atg7 (autophagy‐related

(16) Empagliflozin improves renal injury of diabetic nephropathy complicated with hyperuricemia through AMPK by promoting autophagy and inhibiting apoptosisSaudi Pharmaceutical JournalFebruary 1, 2026Zhiqin Zhang, Yanmei Xu, Chuanwen Xu, Qi Gao, Xiaoyan Wu, Ting WuRabbit monoclonal phospho-AMPK antibody (cat.no.2535; CST; USA), Rabbit monoclonal AMPK antibody (cat.no.5831; CST; USA), Rabbit monoclonal LC3A/B antibody (cat.no.12741; CST; USA), Rabbit monoclonal Cleaved Caspase-3

(17) Ad-VT Oncolytic Adenovirus Suppresses Bladder Cancer via cAMP-Dependent AMPK-Raptor Activation and G2/M ArrestTumour Virus ResearchJanuary 29, 2026Dapeng Li, Jing Lu, Ran Zhu, Xianyan Sun, Cuiling Zhang, Mingzhe Sun, Chengyuan Ma, Chao Shang, Xiao Limedium. The primary antibodies, CyclinB1 (#4131), p-cdc2 (#4539), cdc2 (#9116), p21 (#2947), AMPK (#5831), p-AMPK (#2535), Raptor (#2280), p-Raptor (#2083), mTOR (#2983), p-mTOR (#5536), S6 (#2317), p-S6 (#4858),

(18) EZH2 directs HER2+ breast cancer progression through the modulation of epithelial plasticityEMBO ReportsJanuary 28, 2026Linshan Liu, Ellie J Massey, Dongmei Zuo, Alain Pacis, Mert Demirdizen, Elizabeth Podleszanski, Jessica Cinkornpumin, Yu Gu, Hailey Proud, Virginie Sanguin-Gendreau, et al.,Cell Signaling,9102 Rabbit anti-p-Erk1/2 (T202/Y204),Cell Signaling,4370 Rabbit anti-Ampk,Cell Signaling,5831 Rabbit anti-p-Ampk (T172),Cell Signaling,2535 Mouse anti-pan Akt,Cell Signaling,2920 Rabbit anti-p-Akt

(19) α-Lipoic Acid Alleviates Non-Alcoholic Fatty Liver Disease by Elevating Chaperone-Mediated Autophagy and Increasing β-Oxidation via AMPK-TFEB AxisNutrientsJanuary 26, 2026Keting Dong, Miao Zhang, Jiaojiao Xu, Xue Bai, Jianhong Yangpurchased from Cell Signaling Technology (Danvers, MA, USA) were anti-AMPKα (D5A2) rabbit mAb (1:2000; 5831), anti-Phospho-AMPKα (Thr172) (40H9) Rabbit mAb (1:2000; 2535) and SOD2 (1:2000; D3X8F) XP® Rabbit mAb.

(20) ATF4 regulates mitochondrial dysfunction and mitophagy, contributing to corneal endothelial apoptosisScientific ReportsJanuary 21, 2026Saba Qureshi, Stefan Y. Kim, Stephanie Lee, Lukas Ritzer, William Steidl, Gayle Joliet Krest, Anisha Kasi, Varun Kumar5536, mTOR cat no. 2983, P-Akt cat no. 4060, Akt cat no. 4691, P-AMPKα cat no. 2535, AMPKα cat no. 5831, Actin cat no. 3700; all from Cell Signaling; except Tim23 from Proteintech) overnight at 4 °C. The

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AMPKalpha (D5A2) Rabbit mAb

AMPKalpha (D5A2) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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AMPKalpha (D5A2) Rabbit mAb from Cell Signaling Technology

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