For immunofluorescence analysis, Raji cells were fixed and permeabilized for detection of endogenous POU2AF1 using Anti-POU2AF1 Recombinant Rabbit Monoclonal Antibody (Product # 703514, 1:100) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938), and Rhodamine Phalloidin (Product # R415, 1:300) was used for cytoskeletal F-actin (red) staining. Panel a-d) clearly demonstrates predominant nuclear localization of POU2AF1 in Raji cells. Panel e-h) shows no specific staining in Jurkat cells which is POU2AF1 negative, demonstrating specificity. The images were captured at 60X magnification.