Western blot analysis of RUNX1+RUNX2+RUNX3 on different lysates with Rabbit anti-RUNX1+RUNX2+RUNX3 antibody (ET1612-49) at 1/1,000 dilution. Lane 1: Saos-2 cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: SHG-44 cell lysate Lane 4: Mouse thymus tissue lysate Lysates/proteins at 10 µg/Lane1-3 and 20ug/Lane4. Predicted band size: 49 kDa Observed band size: 50 kDa Exposure time: 14 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-49) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.