Western blot analysis of CD3D on Jurkat cell lysates with Rabbit anti-CD3D antibody (ET1701-57) at 1/1,000 dilution. Lysates/proteins at 15 µg/Lane. Predicted band size: 19 kDa Observed band size: 23 kDa Exposure time: 17 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-57) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.