Western blot analysis of MBP in, Lane 1: mouse brain tissue lysates, Lane 2: rat brain tissue lysates, . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. The membrane was blocked with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with MBP Polyclonal Antibody (Product # PA5-79642) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit. A specific band was detected for MBP at approximately 17-33KD. The expected band size for MBP is at 17-33KD.

Immunohistochemistry analysis of MBP on paraffin-embedded rat brain tissue. Sample was incubated with MBP polyclonal antibody (Product# PA5-79642).