Immunohistochemistry analysis of PML on paraffin-embedded mouse spleen tissue. Sample was incubated with PML polyclonal antibody (Product# PA5-79836) with a dilution of 1 µg/mL, and developed by Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.

Western blot was performed using Anti-PML Polyclonal Antibody (Product # PA5-79836) and a 75 kDa band corresponding to PML was observed in all tested cell lines lysates, and was upregulated upon IFNa treatment in HeLa. Modified whole cell lysates (1% SDS) (30 µg lysate) of HeLa (Lane 1), HeLa treated with IFNa (1000U, 8 hours) (Lane 2), A549 (Lane 3) and SK-OV-3 (Lane 4) were electrophoresed using NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blots were probed with the primary antibody (0.5 µg/mL concentration) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).