Immunocytochemistry/Immunofluorescence analysis of TCP1 in CACO-2 cells using TCP1 Polyclonal Antibody (Product # PA5-80100). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL. DyLight 488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Immunohistochemistry analysis of TCP1 on paraffin-embedded human testis tissue. Sample was incubated with TCP1 polyclonal antibody (Product# PA5-80100).
Western blot analysis of TCP1 in Lane 1: rat brain tissue lysate, Lane 2: rat testis tissue lysate, Lane 3: mouse spleen tissue lysate, Lane 4: mouse thymus tissue lysate, Lane 5: HeLa whole cell lysate, Lane 6: MCF-7 whole cell lysate using 40-50 µg per well. Sample was incubated with TCP1 (Product # PA5-80100) at a dilution of 0.5 µg/mL.
Supplier Page from Thermo Fisher Scientific for TCP1 Antibody