Fig 1: Evaluation of gonadotoxic damage in grafted ovaries in three different groups: control, 4-HC, and 4-HC + let-7a. (a) Immunofluorescent sections show nuclear labelling by Hoechst (blue) and DNA damaged/apoptotic cells by TUNEL (red), scale bar; 100 µm and (b) magnified images, scale bar; 20 µm. (c) The quantification of damaged cells was performed in at least three different ovarian sections (N = 3) for each experimental group (N = 3). The error bars represent the standard error, *p < 0.05. (d) Hematoxylin & Eosin counterstaining and immunostaining of FAS receptor on 5 µm sections of grafted ovaries in different conditions (N = 2). The apoptotic cells of the ovarian follicles are FAS positively stained, (10×) and magnified images (40×), scale bar; 20 µm.